There are two methods to do miRNART-PCR: stem-loop method and tail-adding method. U6 is very common. You can see other people's ready-made designs by checking the relevant literature yourself. The reverse primer of the tail-adding method is universal, and the forward primer only takes the sequence of U6 and designs it by itself.

The order is as follows:

tomato

U6

small

nuclear

ribonucleic acid

gene

1

ataaatcttt

Tata Tata

gtatatttat

gtaagttttc

acgttgagta

aatagcgaag

6 1

aagttgggcc

caaccaagta

aaataagaag

gccgggccat

tacaattaag

tcgtcacaca

12 1

actgggcttc

attgaaaaaa

gcgcaaaaacc

gattccaggc

ccgtgttagc

atgaagactc

18 1

aactcaacca

gagatttctc

cctcatcgct

tacagaaaaa

agctatatgc

tgtttatatt

24 1

gcgaatctaa

cagtgtagtt

tgtcccttcg

gggacatccg

ataaaattgg

aacgatacag

30 1

Agata ge

catggcccct

gcgcaaggat

Gakaka

aatcgagaaa

tggtccaaaa

36 1

ttttttttgc

cattcttttc

aagctccatt

gtcaaatttt

cggggggttt

tgaagtcgcc

42 1

tatctgaggt

tagtctctct

gcatctgatc

agtctgaaac

tttagtcgct

gagcttattc

48 1

tttgttctgg

acttgaagat

taggtcaaat

gattaggttt

atctttcaat

Tata Tata

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