Grafting method of lemon

Lemon tree 1 grafting propagation, cutting: common cutting in spring. The grafting effect is better when the sap begins to flow. Sichuan and Chongqing can be grafted in the first half of rainy days, and Guangdong has a high survival rate within 40 days after beginning of spring. In years with low temperature, the grafting time can be postponed to May. Cutting operation is simple, germination is fast and growth is fast. It can meet the specifications of the nursery in that year and can be planted in autumn or the following spring.

2. Abdominal grafting: Abdominal grafting is generally used for autumn grafting, which can be carried out in the first half of the rainy season to 1 1. The survival rate of abdominal transplantation is high, and it can be supplemented 2 ~ 3 times without transplantation once. The cutting method of scion is the same as that of cutting. At a distance of about 10 cm from the ground, glossy surface is selected to cut longitudinally from top to bottom, with a length of about 2 cm, and the cutting depth is appropriate to not damage or slightly damage xylem. Immediately after opening, insert the scion and tie the opening with plastic film strips from bottom to top.

3. Bud grafting: Bud grafting can be divided into two types: small bud grafting and small bud grafting. Bud cutting is mainly carried out in spring, and bud abdominal grafting is mainly carried out in autumn. Fast budding speed, high working efficiency, small rootstock wound and easy healing. When cutting buds, turn the scion upside down with your left hand, hold the knife with your right hand, and the blade should be flat. Cut from the lower end of the bud to the upper end of the bud until it is 0.5 cm higher than the bud, so that the bud has a slight xylem. Then cut the scion vertically downward with a knife and take off the bud.

Tissue culture of lemon tree The sterilized shoot tips were cut into 5 ~ 8 mm long, then kept polar and inoculated on callus induction medium. After 3 ~ 4 weeks of culture, the explants began to form callus. At this time, it can be cut off and transferred to bud induction medium. After several weeks of culture, buds can be formed, which can be cut off and transferred to root induction medium. After 3 ~ 4 weeks of culture, regenerated plants with roots can be formed. Culture temperature is 24 ~ 26℃, illumination is 8 ~ 12 hours/day, and illumination 1000 ~ 2000 lux.

References:

Lemon Tree Breeding-Baidu Encyclopedia