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Target-HIC series hydrophobic interaction chromatographic columns
Packaging overview:

? Target-HIC series hydrophobic interaction chromatographic columns are specially designed and developed for efficient separation of protein, oligonucleotides and peptides. As shown in the following table, Target-HIC series consists of two ligands (butyl and phenyl) with different hydrophobicity from low to high, which are bonded to non-porous PS/DVB filler through butyl.

Non-porous hydrophobic packing with narrow particle size distribution can effectively separate protein, such as monoclonal antibodies (MAb), antibody-coupled drugs (ADC) and related protein fragments of DNA and oligonucleotides.

Characteristics of Target-HIC series hydrophobic interaction chromatographic columns;

● ? Monodisperse particle packing has the characteristics of high column efficiency and high resolution?

● ? Three particle sizes:1.7,5, 10? M, meet the needs of UPLC, HPLC analysis and semi-preparation?

● ? Wide pH range: 2 ~ 12

● ? High pressure resistance: 1.7 and 5, 10? M-size packing can withstand 8000 psi and 5000 psi respectively?

● ? It is especially suitable for the separation and analysis of monoclonal antibodies and their antibody conjugates (ADC)

Excellent durability and stability:

Chromatographic conditions:

Chromatographic column: Target-HICb 5um 4.6*50mm.

Mobile phase: a (0. 1m sodium solution containing 2m ammonium sulfate.

Phosphate, pH7.0), B(0. 1M phosphate pH7.0)

Gradual change: 0-15min (0- 100% b),15-20min (100% b); ?

Flow rate: 0.4 ml/min

Detector: UV @ 2 14 nm.

Column temperature: 25℃

Sample: ovalbumin (1.0mg/ml),

Chymotrypsin (0.5 mg/ml),

Sample size: 4ul

Excellent reproducibility between batches:

Chromatographic conditions:

Chromatographic column: Target-HICb 5um 4.6*50mm.

Mobile phase: a (0.025m sodium solution containing 2m ammonium sulfate.

Phosphate, pH7.0), B(0.025M sodium phosphate, pH7.0), C(IPA, 100%).

Flow rate: 0.5ml/min.

Detector: UV @ 2 14 nm.

Column temperature: 25℃

Sample: ADC in 0.025m sodium phosphate (1.0mg/ml).

Sample size: 10ul

Order information:

If necessary, please search Chengdu Moore Scientific Instrument Co., Ltd..