New industries in Shenzhen (semi-automatic semi-finished products, fully automatic listing recently, rising day by day).

Beijing Yuan De (there are a large number of semi-automatic equipment in China, and fully automatic equipment is also listed in 12).

Beijing Beiaikang (a domestic model of magnetic particle luminescence method, which has been acquired by Yuan De).

Beijing, Ke Mei and East Asia (semi-automatic is in the middle level, and full-automatic is only the combination of full-automatic sampling and semi-automatic lighting, not full-automatic in the true sense, and the market is very general at present).

Xinbo (the representative of domestic time-resolved luminescence, "Guangzhou Fenghua is still doing time resolution, but the market performance is not as good as Xinbo", and the domestic market of hepatitis B project is doing very well and is currently acquired by PE).

Zhengzhou Antu (Antu has a general biotechnology foundation, but its market-making ability is first-class in China! The equipment level is average, but the parameters are good. At present, the overall performance of the market is good, fully automatic will be listed soon).

Weihai Gaowei (semi-automatic is very poor, 200-speed automatic has been listed recently, and the equipment is large and beautiful, but the reagent situation and actual use are not known, and the overall market performance is average at present).

Beijing Taige Kexin, an old-fashioned luminous manufacturer (its reagent batch number is very complete, and even the batch number can't make the reagent! The technical level is average, the market is chaotic, and it is getting lower and lower).

Sichuan Mike (newly listed fully automatic, but there are few kinds of reagents! I haven't met the user yet).

There are also some small manufacturers, such as Dacheng in Beijing and Kangpusheng in Hebei (only producing equipment).

Classification:

Chemiluminescence labeling immunoassay;

Chemiluminescence labeling immunoassay, also known as chemiluminescence immunoassay (CL IA), is an immunoassay method that directly labels antigens or antibodies with chemiluminescence reagents. Acridine ester (AE) is an acridine ester compound commonly used for labeling, and it is an effective luminescent marker [3]. It can complete intense direct luminescence within one second by starting the luminescent reagent (N ao H H H 2O 2), which belongs to fast scintillation luminescence (see figure 1). Acridine ester is used as a marker of immunoassay, and its chemical reaction is simple and rapid without catalyst. The competition method is used to detect small molecule antigen, and the sandwich method is used to detect large molecule antibody, which has less non-specific binding and low background. Binding with macromolecules will not reduce the amount of light generated, thus increasing sensitivity.

Luminase immunoassay:

From the point of view of labeled immunoassay, chemiluminescence enzyme immunoassay (CLEIA) belongs to enzyme immunoassay, except that the substrate of enzyme reaction is luminescent agent, and the operation steps are exactly the same as those of enzyme immunoassay [5]: the immune reaction is carried out by using bioactive substances (such as enzyme-labeled antigens or antibodies), and the enzyme on the immune reaction complex acts on the luminescent substrate and the signal reagent. At present, the commonly used marker enzymes are horseradish peroxidase and alkaline phosphatase, both of which have their own luminescent substrates.