Carnot fixative has high permeability, which is suitable for the fixation of general plant tissues and cells, and is often used for root tip and anther tabletting. After fixation, it should be washed with 95% alcohol until it contains no glacial acetic acid.

The experiment of observing apical mitosis in high school textbooks can actually be fixed with Kano solution, but it is not necessary.

Because hydrochloric acid and alcohol are added when the root tip is free, hydrochloric acid plays a free role and alcohol plays a fixed role. In this experiment, alcohol fixation has played a very good role, so Carnot solution is not needed.

In fact, Carnot liquid plays the biggest role in fixing, which is actually alcohol:

3 parts anhydrous alcohol: glacial acetic acid 1 part.

Or 6 parts of absolute ethanol, 3 parts of chloroform (toxic) and glacial acetic acid (acetic acid) 1 part.