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Wedding tga template
This is a coding chain. The design of primers is influenced by the use of cross-chains, because the nucleotide structure is asymmetric and divided into three ends and five ends.

Therefore, 5'-ATCG XXXXXXXXXXXX and 3'-ATCG XXXXXXXXXXXX are two different primer molecules.

Primer design software uses coding chain, so the landlord doesn't have to bother to copy and paste directly.