Follicle puncture is widely used. For example, some women suffer from endometriosis or luteinized unruptured follicle syndrome. These two special cases are that a good egg can't come out of the follicle and sperm can't combine with the egg. Follicle puncture plays an auxiliary role in the combination of sperm and egg, that is, under the guidance of transvaginal ultrasound, follicular rupture, follicular fluid flows out and the egg comes out, so there are more opportunities for sperm and egg to combine. This is the application range of follicular puncture without IVF. Another situation is that IVF takes eggs. When women enter the procedure of artificial assisted technology and use some ovulation drugs to make the eggs more and more mature, we will puncture the follicles through vagina to get the eggs and put them in a Petri dish for cultivation and fertilization. Sperm and egg are combined with in vitro fertilization, and the small embryo is raised to the mother three to five days after combination. Therefore, in order to successfully conceive IVF, you have to get the seed, and the woman's seed is the egg. Eggs can only be obtained by ovarian puncture and follicular aspiration.