When the flowers wither, the foot buds will come out. When the foot buds come out, they will cut off the main branches and choose strong foot buds to cultivate new plants. Old plants can only be used as the basis for bud selection and reproduction. Pay attention to the old plants and old soil can no longer be used, otherwise it will be bad to grow old.

1, chrysanthemum (Chry santhemum mori f olium) is a perennial plant of Chrysanthemum in Compositae, and it is one of many varieties of chrysanthemum. Leaves are solitary and alternate, dark green in color, oval to oblong, with concave edges, many stems, peanuts at the top of branches, and flowering for 2 months (mid-August to mid-June 10).

2. Because a flower will bloom on each rhizome, a new stem will grow on the old stem after flowering, and the new stem will bloom again, so a pot of chrysanthemum can bloom many flowers, so it is called chrysanthemum with a thousand heads. Because of its beautiful flower posture, diverse colors and other excellent quality characteristics, it has high ornamental value and commercial value; At the same time, it has important medicinal value.

3. In Compendium of Materia Medica, there is a discussion that "Chrysanthemum will benefit qi and blood for a long time, improve intelligence and fitness, and resist aging and prolong life". The asexual propagation of chrysanthemum usually adopts cutting, but this method has low propagation index and long cycle, which is difficult to meet the requirements of mass production.

4. The optimum medium for shoot tip induction of Chrysanthemum morifolium is M S+6-BA2.0mg/L, and the callus induction rate is 965438 0.3%. The optimum differentiation and proliferation medium for plant regeneration is1/2m s (iron salt content unchanged). Callus transferred to this medium differentiated into green buds after 7 ~ 10d, and the seedling rate reached 79.2% after 40~ 50d.

5. In order to successfully apply the tissue culture method to production practice, the following points need to be met: (1) has a high proliferation index. (2) The formation time of buds and roots is short, and the growth cycle is short. (3) The seedlings are robust and the survival rate is high when transplanted. (4) Minimize the dosage of hormones and expensive drugs. Compared with the conventional method, this method can effectively reduce the production cost and shorten the production cycle.