One-step homologous recombination is a technique of seamless cloning based on the principle of homologous recombination. This technique does not need to consider restriction sites, and is suitable for directional cloning of almost any vector and any fragment. The operation process is simple and rapid, and the reaction can be completed in 20 minutes.

One-step homologous recombination is a technique of seamless cloning based on the principle of homologous recombination. This technique does not need to consider restriction sites, and is suitable for directional cloning of almost any vector and any fragment. The operation process is simple and rapid, and the reaction can be completed in 20 minutes.