95% alcohol fixation and 1% formaldehyde fixation are two different chemicals, and their fixation methods and functions are also different. 95% alcohol fixation is a common cell fixation method, which can fix protein, nucleic acid and other molecules in the cell membrane and cells in the cell to prevent them from changing in the subsequent staining and observation process. 95% alcohol fixation can keep the cell morphology intact and is suitable for the fixation of most cell types. Formaldehyde fixation is a common method of tissue fixation, which can fix protein, nucleic acid and other molecules in the tissue to prevent them from changing in the subsequent staining and observation. Formaldehyde fixation can keep the tissue structure intact and is suitable for the fixation of most tissue types. Therefore, the difference between 95% alcohol fixation and 1% formaldehyde fixation lies in their different fixation objects and fixation methods, which are suitable for different types of samples.