Stem cell preservation refers to the separation and cultivation of stem cells from different human tissues, which are frozen and preserved at-196℃ after detection and identification, so that the stem cells can be revived when needed in clinic and used for reinfusion to patients to achieve the purpose of treating diseases. So is it necessary to store stem cells? The benefits of stem cell storage mainly include:
1. Storing cells is not only a health guarantee, but also a health investment, which can be used conveniently when stem cell therapy is needed in the future.
2. Umbilical cord mesenchymal stem cells do not need to be matched between family members, and the effect is good. It only needs to store stem cells from newborn umbilical cord, which can be used by the whole family.
3. Store healthy and energetic stem cells in advance, which can be used for health care, anti-aging and disease treatment in the future.
4. Stored stem cells can reserve life resources for future breakthroughs in stem cell research.
Generally speaking, the storage of stem cells plays a certain role. Stem cells extracted from umbilical cord of newborns are generally stored and will not affect the health of infants. Consider storage if necessary.
Second, how to store stem cells?
The preservation method of stem cells is not complicated, and the steps are as follows:
1. collection: extract hematopoietic stem cells from umbilical cord and placenta of newborn, or extract adult adipose stem cells from peripheral blood.
2. Production: After the collected blood is centrifuged, the nucleated cells are collected and cultured in a special culture medium for hematopoietic stem cells, so that the nucleated cells are reversed into hematopoietic stem cells.
3. Detection: Bacterial detection, cell counting and flow cytometry were carried out on the 3rd and 6th day of stem cell production to ensure that the stem cell production process was not polluted and the number of stem cells increased steadily.
4. Preservation: The stem cells were collected from the 6th day of production, packed in 2ml freezing tubes, cooled to -80℃ and stored in a liquid nitrogen tank at-196℃ according to the preservation requirements.
Third, which stem cells can be preserved?
Storing stem cells is a popular way. There are many kinds of stem cells, but not all of them are worth preserving. There are three main types of stem cells suitable for storage:
1, umbilical mesenchymal stem cells
Umbilical cord mesenchymal stem cells are multifunctional stem cells existing in neonatal umbilical cord tissue. Mesenchymal stem cells are abundant and have strong differentiation ability. One umbilical cord stem cell can be used for many times, and can be directly used by the whole family, with broad application prospects.
2. Adipose stem cells
Adipose stem cells have the advantages of multiple collection, high yield per unit volume and convenient collection. Adult stem cells are all differentiated from adipose-derived stem cells, which are mostly used in clinical applications such as disease treatment, plastic surgery and tissue engineering.
3. Umbilical cord blood hematopoietic stem cells
Umbilical cord blood refers to the blood left in the placenta and umbilical cord after the fetus is delivered, the umbilical cord is ligated and cut off, which contains a large number of hematopoietic stem cells. The collection of umbilical cord blood will not cause any harm to infants and parturients, which is more convenient.