Women only ovulate one egg every month. It is not enough to make one egg in a test tube. Drugs are needed to make patients ovulate multiple eggs in a week.

Painless egg retrieval

Collect eggs at a certain time (B-ultrasound monitors follicular development and determines the best time to induce ovulation by giving hormones). The methods of taking eggs include: puncture of follicles under laparotomy, abdominal wall taking eggs under the guidance of B-ultrasound, laparoscopic taking eggs, vaginal puncture taking eggs or taking eggs from urethra and bladder.

① exploratory laparotomy to take eggs.

The method adopted in the early days. Its advantage is that it can correct some diseases at the same time in open surgery, such as tubal separation and recanalization, and directly extract follicular fluid by selecting suitable eggs under direct vision. Simple operation and high egg collection rate. However, the operation is traumatic and costly.

② Laparoscopic egg retrieval: This method is the most widely used. Under laparoscope, ovarian follicles can be punctured by intubation to take eggs, and other pelvic lesions can be observed at the same time.

Some feasible operations were performed, and the surgical injury was small. However, laparoscopic equipment and technology are needed, and intraperitoneal injection of co (carbon dioxide) will reduce the fertilization ability of eggs.

③b-ultrasound-guided transvaginal egg retrieval: it is performed after midnight puncture. This method is particularly simple, safe, does not require hospitalization, and saves money. However, the instrument is expensive, the operation technology is a bit complicated, and there are not many follicles punctured under direct vision.

Egg culture

After sucking out follicular fluid, look for eggs immediately. After the egg is taken out, it needs to be placed in a Petri dish, which contains artificial culture solution and a certain amount of follicular fluid, which is helpful for the growth and development of the egg. The culture time depends on the maturity of follicles, and mature follicles are usually cultured for 2 ~ 6 hours.

embryo transplantation

Wash vagina and cervix with normal saline, then scrub with culture solution, and put metal or plastic catheter into cervical canal. Culture medium, insert this tube into the cervical catheter and place it 0.5cm away from the uterine bottom, and send the embryo into the uterine cavity. After embryo transfer, you need to stay in bed for 24 hours, limit your activities for 3 ~ 4 days, and receive progesterone treatment. Two weeks after transplantation, β-GCG should be determined. If you are not pregnant, you should stop using progesterone.

external fertilization

After collecting semen, semen was obtained in vitro by washing method, and then it was put into the culture solution containing oocytes, cultured for 65438±05 ~ 24h, and examined under dissecting microscope. If two pronuclei are found in the protoplasm of oocytes, it indicates that fertilization has been carried out, and then continue to culture for 36 ~ 42 hours. When the fertilized egg has divided into 4 ~ 8 cells under the microscope, embryo transfer can be considered.

embryo transplantation

Take the early embryos out of the culture solution and inject them into the uterine cavity. It is best to transplant 3-4 fertilized eggs at a time, which can improve the success rate of pregnancy after transplantation. The conditions for successful embryo transfer are: the vitality of the fertilized egg itself, whether the endometrium is healthy, and whether there is any damage during the transfer.

Late pregnancy measures

Pregnancy after successful embryo transfer to uterine cavity should be regarded as high-risk pregnancy. Follow up closely, do all kinds of necessary hormone determination and routine examination, and take measures to protect the fetus. If you are in Kunming, it is recommended to go to Kunming and Wanjia Hospital to do test tubes professionally. A relative of mine did it there.